| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Minnella, Mary J (8)
|
NIPACK |
25.73 |
26.71 |
0.98 |
| 2 |
Magnera, Alaura (8)
|
NIWEBS |
30.58 |
30.15 |
-0.43 |
| 3 |
Yu, Emily X (8)
|
NIPACK |
33.00 |
31.93 |
-1.07 |
| 4 |
Brett, Catherine M (7)
|
NIFAST |
33.23 |
34.58 |
1.35 |
| 5 |
Hiller, Rachel J (6)
|
NIPACK |
|
36.62 |
- |
| 6 |
Gonzalez, Alexis W (8)
|
NIPACK |
34.51 |
37.51 |
3.00 |
| 7 |
Van Hout, Kennyn A (8)
|
NISDSC |
34.98 |
37.57 |
2.59 |
| 8 |
Boyum, Paige L (8)
|
NIFAST |
37.06 |
38.00 |
0.94 |
|
Sullivan, Brynn E (7)
|
NIFAST |
37.91 |
DQ |
- |
|
McGahan, Molly E (7)
|
NIIRON |
33.84 |
DQ |
- |
|
Dellis, Julia M (6)
|
NIPACK |
|
DQ |
- |
|
Donnelly, Elizabeth G (6)
|
NIPACK |
|
DQ |
- |
|
Patel, Soha M (6)
|
NIPACK |
|
DQ |
- |
|
Marsh, Olivia D (6)
|
NIWEBS |
|
DQ |
- |
|
Farrell, Kamryn L (6)
|
NIGRSC |
34.97 |
NS |
- |