| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Sullivan, Maeve C (10)
|
NIFAST |
54.87 |
51.58 |
-3.29 |
| 2 |
Brett, Erin E (9)
|
NIFAST |
52.76 |
51.72 |
-1.04 |
| 3 |
Munson, Sophie M (9)
|
NIGRSC |
55.40 |
52.23 |
-3.17 |
| 4 |
Joshi, Reva R (10)
|
NIPACK |
58.61 |
53.70 |
-4.91 |
| 5 |
Malik, Vani (9)
|
NIWEBS |
|
54.31 |
- |
| 6 |
Man, Shaila T (10)
|
NIPACK |
56.00 |
56.16 |
0.16 |
| 7 |
Degreve, Ella A (10)
|
NIWEBS |
54.51 |
56.17 |
1.66 |
| 8 |
Man, Surya T (9)
|
NIPACK |
|
56.47 |
- |
| 9 |
Inzinga, Rose Marie (10)
|
NIWEBS |
|
58.53 |
- |
| 10 |
VanArsdale, Leah M (9)
|
NIWEBS |
1:00.98 |
59.67 |
-1.31 |
| 11 |
Huerta-Andrade, Amanda (9)
|
NIFAST |
1:00.30 |
1:00.97 |
0.67 |
| 12 |
van Wichen, Jessica D (9)
|
NIWEBS |
1:00.47 |
1:01.77 |
1.30 |
| 13 |
Brown, Rhea R (9)
|
NIVICT |
1:05.93 |
1:03.69 |
-2.24 |
| 14 |
Rist, Olivia B (10)
|
NIHAST |
1:05.38 |
1:04.84 |
-0.54 |
| 15 |
Thayse, Eden A (9)
|
NISDSC |
1:11.76 |
1:06.10 |
-5.66 |
| 16 |
Brodt, Hannah C (9)
|
NIWEBS |
1:13.83 |
1:09.94 |
-3.89 |
| 17 |
Fortuna, Katelyn G (9)
|
NIFAST |
1:10.03 |
1:12.07 |
2.04 |
|
Brockman, Reese S (10)
|
NIRRST |
1:03.94 |
DQ |
- |
|
Hopkins, Elise R (9)
|
NIGRSC |
|
DQ |
- |