| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Cassidy, Alexa E (12)
|
NISDSC |
|
2:46.06 |
- |
| 2 |
Magnera, Sofia A (11)
|
NIWEBS |
2:57.66 |
2:49.76 |
-7.90 |
| 3 |
Christodaro, Raena M (12)
|
NIHAST |
3:04.28 |
2:53.17 |
-11.11 |
| 4 |
Vazquez Serra, Covadonga (12)
|
NIPACK |
3:00.47 |
2:54.87 |
-5.60 |
| 5 |
LaChance, Carmindy (11)
|
NIIRON |
3:10.38 |
2:55.24 |
-15.14 |
| 6 |
Bartholomay, Meghan E (11)
|
NIGCOM |
2:57.36 |
2:57.19 |
-0.17 |
| 7 |
Carey, Madison H (11)
|
NIIRON |
2:59.57 |
2:59.38 |
-0.19 |
| 8 |
Werner, Riley K (12)
|
NIVICT |
|
3:09.10 |
- |
| 9 |
Thoen, Sarah G (11)
|
NISDSC |
|
3:17.29 |
- |
| 10 |
Girgis, Sophie G (11)
|
NIPACK |
3:44.48 |
3:40.60 |
-3.88 |
| 11 |
Cady, Makenna N (11)
|
NIWEBS |
4:11.64 |
4:01.72 |
-9.92 |
| 12 |
Cabral, Aurora (11)
|
NIRRST |
4:01.66 |
4:19.12 |
17.46 |