| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Mattle, Skye M (10)
|
NISDSC |
47.36 |
46.46 |
-0.90 |
| 2 |
Phillips, Hayley Q (9)
|
NIIRON |
47.11 |
47.36 |
0.25 |
| 3 |
Man, Shaila T (10)
|
NIPACK |
48.40 |
47.61 |
-0.79 |
| 4 |
Brett, Erin E (9)
|
NIFAST |
49.08 |
48.06 |
-1.02 |
| 5 |
Bhargava, Anya (10)
|
NIVICT |
47.34 |
48.61 |
1.27 |
| 6 |
Galley, Alyssa E (9)
|
NIFAST |
51.28 |
48.62 |
-2.66 |
| 7 |
van Wichen, Jessica D (9)
|
NIWEBS |
52.81 |
48.80 |
-4.01 |
| 7 |
Wagner, Keaghan J (9)
|
NISDSC |
48.75 |
48.80 |
0.05 |
| 9 |
Malik, Vani (9)
|
NIWEBS |
58.50 |
50.33 |
-8.17 |
| 10 |
Man, Surya T (9)
|
NIPACK |
55.17 |
50.42 |
-4.75 |
| 11 |
Munson, Sophie M (9)
|
NIGRSC |
56.02 |
50.59 |
-5.43 |
| 12 |
Thayse, Eden A (9)
|
NISDSC |
55.91 |
50.85 |
-5.06 |
| 13 |
Sullivan, Maeve C (10)
|
NIFAST |
53.04 |
51.34 |
-1.70 |
| 14 |
Olbrys, Lilja (10)
|
NIVICT |
51.19 |
52.30 |
1.11 |
| 15 |
Koneski Collette, Eliana J (10)
|
NIGRSC |
51.21 |
52.64 |
1.43 |
| 16 |
Hackett, Grace C (10)
|
NIIRON |
55.35 |
53.51 |
-1.84 |
| 17 |
VanArsdale, Leah M (9)
|
NIWEBS |
56.37 |
54.73 |
-1.64 |
| 18 |
Inzinga, Rose Marie (10)
|
NIWEBS |
|
55.06 |
- |
| 19 |
Huerta-Andrade, Amanda (9)
|
NIFAST |
51.78 |
55.07 |
3.29 |
| 20 |
Nhaila, Salma (10)
|
NIVICT |
58.03 |
55.12 |
-2.91 |
| 21 |
Bub, Emily R (9)
|
NIGCOM |
56.94 |
57.67 |
0.73 |
| 22 |
Brown, Rhea R (9)
|
NIVICT |
1:00.73 |
58.19 |
-2.54 |
| 23 |
Thurston, Lauren A (10)
|
NIGCOM |
59.05 |
58.90 |
-0.15 |
| 24 |
Brockman, Reese S (10)
|
NIRRST |
1:00.83 |
59.23 |
-1.60 |
| 25 |
Fortuna, Katelyn G (9)
|
NIFAST |
59.94 |
1:02.70 |
2.76 |
| 26 |
Galley, Morgan C (9)
|
NIRRST |
1:01.67 |
1:05.16 |
3.49 |
| 27 |
Hopkins, Elise R (9)
|
NIGRSC |
|
1:10.93 |
- |
|
Zebrak, Madeleine E (9)
|
NISDSC |
56.53 |
DQ |
- |
|
Kalbfus, Taylor R (9)
|
NIFAST |
48.09 |
DQ |
- |