| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Smith, Logan (17)
|
ZZCANY |
23.78 |
24.48 |
0.70 |
| 2 |
Jung, Collin S (14)
|
ILHEAT |
24.36 |
24.67 |
0.31 |
| 3 |
Hammond, James (15)
|
ZZCANY |
25.34 |
24.90 |
-0.44 |
| 4 |
Wirebaugh, William (14)
|
WCY |
25.95 |
26.74 |
0.79 |
| 5 |
Risby, Simeon T (14)
|
ILYDSC |
26.42 |
27.10 |
0.68 |
| 6 |
Vickroy, Cullen (15)
|
WCY |
27.68 |
27.65 |
-0.03 |
| 7 |
Johnson, Kyle K (13)
|
ILHEAT |
27.41 |
28.25 |
0.84 |
| 8 |
Rienstra, Nicholas M (13)
|
ILHEAT |
28.12 |
28.31 |
0.19 |
| 9 |
Johnson, Joshua (16)
|
ILCLY |
28.36 |
28.46 |
0.10 |
| 10 |
Geissler, Alex T (14)
|
ILHEAT |
26.77 |
28.55 |
1.78 |
| 11 |
Brewster, William (13)
|
ILKNCY |
28.57 |
29.01 |
0.44 |
| 12 |
Goodman, Markus (14)
|
ILCCY |
28.56 |
29.04 |
0.48 |
| 13 |
Goodman, Nicholas (16)
|
ILCCY |
28.72 |
29.58 |
0.86 |
| 14 |
Mattan, John (13)
|
WCY |
30.80 |
30.12 |
-0.68 |
| 15 |
Foster, Luke (13)
|
ILKNCY |
31.30 |
30.47 |
-0.83 |
| 16 |
Main, Ethan (13)
|
ILKNCY |
30.43 |
30.58 |
0.15 |
| 17 |
Mowen, Gavin (16)
|
ZZCANY |
29.44 |
30.68 |
1.24 |
| 18 |
Dempsey, Maddox C (13)
|
ILHEAT |
28.79 |
30.70 |
1.91 |
| 19 |
Putman, Tanner (13)
|
ZZCANY |
30.51 |
30.88 |
0.37 |
| 20 |
Ding, William Y (13)
|
ILHEAT |
30.61 |
31.15 |
0.54 |
| 21 |
Vazquez, Jack D (13)
|
ILHEAT |
30.64 |
31.29 |
0.65 |
| 22 |
Miller, Drake (13)
|
ZZCANY |
30.01 |
31.37 |
1.36 |
| 23 |
Satheeshreddy, Jishnu (13)
|
ILBNY |
32.63 |
31.94 |
-0.69 |
| 24 |
Turney, Wynn (18)
|
ILCLY |
32.17 |
34.35 |
2.18 |
|
Thompson, AJ (13)
|
ILKNCY |
34.66 |
DQ |
- |
|
Abbot, Brock (14)
|
ILKNCY |
33.46 |
NS |
- |
|
Baloch, Ayaan (13)
|
ILBNY |
45.90 |
NS |
- |
|
Fujimoto, Joshua B (13)
|
ILBNY |
28.13 |
NS |
- |