| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Cavanagh, Sarah A (12)
|
ILSPY |
29.44 |
29.19 |
-0.25 |
| 2 |
Dohman, Alyse C (11)
|
ILBNY |
28.51 |
29.41 |
0.90 |
| 3 |
McAndrew, Marin B (12)
|
ILHEAT |
28.35 |
29.46 |
1.11 |
| 4 |
Rai, Avani B (12)
|
ILBNY |
29.63 |
29.98 |
0.35 |
| 5 |
Tapper, Kylie (12)
|
ILKNCY |
30.67 |
30.65 |
-0.02 |
| 6 |
Mueller, Anna T (11)
|
ILBNY |
30.97 |
31.11 |
0.14 |
| 7 |
Stambaugh, Emily (11)
|
ZZCANY |
31.53 |
31.33 |
-0.20 |
| 8 |
Skaggs, Tristen (11)
|
WCY |
32.80 |
31.89 |
-0.91 |
| 9 |
Fahnestock, Emma M (12)
|
ILHEAT |
31.55 |
31.94 |
0.39 |
| 10 |
Stewart, Caitlyn (11)
|
WCY |
32.33 |
32.22 |
-0.11 |
| 11 |
Terry, Olivia R (11)
|
ILHEAT |
31.41 |
32.40 |
0.99 |
| 12 |
Miller, Libby (12)
|
WCY |
32.10 |
32.50 |
0.40 |
| 13 |
Mansfield, Madilynn E (12)
|
ILYDSC |
34.69 |
33.35 |
-1.34 |
| 14 |
Wienhoff, Alexa M (12)
|
ILSPY |
34.52 |
33.56 |
-0.96 |
| 15 |
Owen, Olivia (11)
|
ILKNCY |
34.83 |
33.95 |
-0.88 |
| 16 |
Vocks, Addison (12)
|
ILCCY |
33.31 |
34.40 |
1.09 |
| 17 |
Beaty, Madelyn (12)
|
ILCCY |
32.39 |
34.52 |
2.13 |
| 18 |
Warren, Allie (11)
|
ILKNCY |
33.87 |
34.57 |
0.70 |
| 19 |
McQuirter, NaShyla A (11)
|
ILBNY |
34.60 |
35.17 |
0.57 |
| 20 |
Murphy, Rylee (12)
|
ZZCANY |
34.31 |
36.15 |
1.84 |
| 21 |
Khan, Layla A (12)
|
ILHEAT |
34.97 |
36.18 |
1.21 |
| 22 |
Creighton, Rose M (12)
|
ILYDSC |
35.20 |
36.97 |
1.77 |
| 23 |
Vijayakumar, Megha (11)
|
ILYDSC |
36.25 |
37.45 |
1.20 |
| 24 |
Morr, Tessa M (11)
|
ILYDSC |
34.93 |
37.49 |
2.56 |
| 25 |
Vaira, Claire (11)
|
ILCCY |
35.55 |
39.56 |
4.01 |
| 26 |
Harwood, Hannah (11)
|
ILCCY |
41.66 |
43.04 |
1.38 |
| 27 |
Rafferty, Emma (11)
|
ILCCY |
45.93 |
47.85 |
1.92 |
| 28 |
Delgado, Juliana (11)
|
ILCCY |
55.11 |
57.22 |
2.11 |
|
Jung, Chloe K (11)
|
ILHEAT |
32.78 |
NS |
- |
|
Fones, Chloe (11)
|
ILKNCY |
36.96 |
NS |
- |
|
Dempsey, Olivia E (11)
|
ILHEAT |
32.99 |
NS |
- |
|
Foulke, Valerie L (12)
|
ILYDSC |
29.19 |
NS |
- |