| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Perry, Elizabeth M (15)
|
MDHOCO |
|
31.03 |
- |
| 2 |
Descoteau, Morgan D (15)
|
MDCGA |
31.37 |
32.07 |
0.70 |
| 3 |
Plaudis, Haylee K (15)
|
MDCAC |
32.38 |
32.26 |
-0.12 |
| 4 |
Messaris, Charlotte R (15)
|
MDEST |
32.67 |
32.32 |
-0.35 |
| 5 |
Taylor, Emma K (15)
|
MDCGA |
35.54 |
32.67 |
-2.87 |
| 6 |
Mehls, Jenna N (15)
|
MDCAC |
37.08 |
33.08 |
-4.00 |
| 7 |
Fitzsimmons, Norah B (15)
|
MDEST |
33.43 |
33.33 |
-0.10 |
| 8 |
Nagle, Ashleigh M (15)
|
MDCAA |
36.19 |
33.49 |
-2.70 |
| 9 |
Hernan, Maggie (17)
|
MDCATY |
33.04 |
33.65 |
0.61 |
| 10 |
DeSantis, Lauren I (15)
|
MDCGA |
33.68 |
34.34 |
0.66 |
| 11 |
Cole, Olivia C (15)
|
MDCAC |
33.47 |
34.59 |
1.12 |
| 12 |
Oh, Sara S (15)
|
MDHOCO |
36.69 |
36.80 |
0.11 |
| 13 |
Muhammad, Ndera S (15)
|
MDBCSC |
|
45.55 |
- |
|
Stubblefield, Grace M (15)
|
MDCGA |
32.23 |
NS |
- |