| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Steckiel, Emma J (15)
|
MAGMAC |
29.26 |
28.78 |
-0.48 |
| 1 |
Strunk, Skylar R (15)
|
MABYNS |
|
29.39 |
- |
| 1 |
Delaney, Julia J (15)
|
MAPFY |
32.15 |
33.29 |
1.14 |
| 2 |
Glinecke, Emily M (19)
|
AZASU |
29.30 |
30.37 |
1.07 |
| 2 |
Woods, Megan L (15)
|
MAUPY |
35.58 |
37.17 |
1.59 |
| 3 |
Fresa, Noel J (15)
|
MAUPY |
29.83 |
30.74 |
0.91 |
| 4 |
McNulty, Peyton (16)
|
MAPFY |
30.16 |
31.31 |
1.15 |
| 5 |
Megill, Cara E (17)
|
MABYNS |
28.01 |
31.62 |
3.61 |
| 6 |
Draves, Teresa J (16)
|
MATOPS |
31.05 |
32.12 |
1.07 |
| 7 |
Samuels, Madelynne C (15)
|
MAKAY |
31.42 |
33.17 |
1.75 |
| 8 |
Hersh, Michaela B (15)
|
MABYNS |
31.37 |
33.45 |
2.08 |
|
Monaghan, Abaigeal P (15)
|
MAPENN |
|
NS |
- |