| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Marston, Sarah K (15)
|
MALAC |
26.01 |
25.85 |
-0.16 |
| 2 |
Wiglesworth, Katelyn F (15)
|
MALAC |
27.01 |
26.09 |
-0.92 |
| 3 |
Horner, Catherine M (15)
|
MALAC |
26.02 |
26.59 |
0.57 |
| 4 |
Sugra, Anna M (16)
|
MALAC |
|
26.85 |
- |
| 5 |
Nguyen, Ashley M (15)
|
MAHAC |
26.62 |
27.14 |
0.52 |
| 6 |
McCloskey, Grace M (17)
|
MAHAC |
26.53 |
27.93 |
1.40 |
| 7 |
Aluquin, Lindsay A (15)
|
MAHAC |
27.09 |
28.27 |
1.18 |
| 8 |
Fitterer, MayaRose P (15)
|
MAHAC |
29.25 |
28.94 |
-0.31 |
| 9 |
Pedersen, Ella S (15)
|
MAHAC |
27.92 |
29.58 |
1.66 |
| 10 |
Grubb, Kirsten A (15)
|
MAHAC |
30.39 |
30.23 |
-0.16 |
| 11 |
Ruddle, Jillian T (15)
|
MAHAC |
28.96 |
30.46 |
1.50 |
| 12 |
Long, Felicity A (17)
|
MACYA |
35.72 |
35.44 |
-0.28 |