| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Fiore, Mariella S (8)
|
MAUN-0 |
43.08 |
42.20 |
-0.88 |
| 2 |
Hahn, Farrah J (7)
|
MAPSC |
50.14 |
44.57 |
-5.57 |
| 3 |
Darmo, Ally T (8)
|
MAGRA |
55.05 |
48.09 |
-6.96 |
| 4 |
Specht, Hailee T (8)
|
MAPSC |
56.13 |
48.18 |
-7.95 |
| 5 |
LaBaugh, Sydney N (8)
|
MAPSC |
|
48.85 |
- |
| 6 |
May, Grace L (8)
|
MABLUE |
57.62 |
49.86 |
-7.76 |
| 7 |
Laski, Emilia C (8)
|
MAEMAC |
|
50.29 |
- |
| 8 |
Troxell, Lyla B (8)
|
MABLUE |
|
50.49 |
- |
| 9 |
Newhart, Reese E (8)
|
MAEMAC |
|
51.45 |
- |
| 10 |
Shannon, Lia M (8)
|
MABLUE |
1:02.16 |
54.37 |
-7.79 |
| 11 |
Fleischaker, Mazie R (7)
|
MAPAAC |
|
54.95 |
- |
| 12 |
Rojas, Norah J (7)
|
MAPAAC |
|
56.36 |
- |
| 13 |
Chase, Vanessa M (7)
|
MABLUE |
1:11.51 |
59.35 |
-12.16 |
| 14 |
Grimaldi, Ella G (8)
|
MABLUE |
1:07.57 |
59.47 |
-8.10 |
| 15 |
Reddy, Sriya A (7)
|
MAEMAC |
|
1:02.48 |
- |
| 16 |
Porter, Lucille A (7)
|
MAGRA |
|
1:04.62 |
- |
| 17 |
Lyons, Eleanor B (7)
|
MAEMAC |
|
1:05.68 |
- |
| 18 |
Lenhart, Caelee Maye (8)
|
MAEMAC |
|
1:07.85 |
- |
| 19 |
Reddy, Adhira R (7)
|
MAEMAC |
|
1:12.78 |
- |
| 20 |
Lausch, Soledad M (8)
|
MAEMAC |
|
1:14.88 |
- |
| 21 |
Mulvaney, Madeleine X (7)
|
MAEMAC |
|
1:18.87 |
- |
| 22 |
Schellenberger, Elizabeth A (7)
|
MABLUE |
|
1:19.06 |
- |