| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Stokes, Kayden (7)
|
MALYM |
38.89 |
38.24 |
-0.65 |
| 2 |
Peev, Sebastian (8)
|
MAORCA |
45.20 |
39.31 |
-5.89 |
| 3 |
Gonzalez, Nathaniel L (8)
|
MAPENN |
|
41.07 |
- |
| 4 |
Thomas, D'One D (8)
|
MAPENN |
42.98 |
41.55 |
-1.43 |
| 5 |
Del Prim, Nicolas J (8)
|
MAFCA |
44.58 |
41.86 |
-2.72 |
| 6 |
Chernikh, Sergey (6)
|
MAUN |
47.01 |
42.17 |
-4.84 |
| 7 |
Pillar, Ian D (8)
|
MAFCA |
44.05 |
42.90 |
-1.15 |
| 8 |
Litton, Jacob G (8)
|
MAFCA |
45.10 |
43.44 |
-1.66 |
| 9 |
Rimler, Maack S (6)
|
MATRI |
49.84 |
44.18 |
-5.66 |
| 10 |
Zingarelli, Rocco E (8)
|
MAFSS |
|
49.36 |
- |
| 11 |
Rakitskiy-Smetana, Aviel (8)
|
MAORCA |
49.40 |
50.82 |
1.42 |
| 12 |
Kavka, Oleksandr (8)
|
MAORCA |
52.70 |
51.33 |
-1.37 |
| 13 |
Nelson, James T (8)
|
MATRI |
53.41 |
51.50 |
-1.91 |
| 14 |
Paraskevov, Edward (8)
|
MAORCA |
55.60 |
56.53 |
0.93 |
| 15 |
Jung, Maxwell (8)
|
MAFSS |
|
1:00.05 |
- |