| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Carter, Camryn B (16)
|
MAGRA |
52.11 |
54.24 |
2.13 |
| 2 |
Savory, Alexandra M (16)
|
MAUN-3 |
56.11 |
57.35 |
1.24 |
| 3 |
Reynolds, Hope W (17)
|
MAGRA |
55.09 |
57.53 |
2.44 |
| 4 |
Burlein, Hannah J (17)
|
MAGSY |
|
57.72 |
- |
| 5 |
Russell, Grace C (17)
|
MAKAY |
56.76 |
59.55 |
2.79 |
| 6 |
Duncan, Marlies A (17)
|
MACAA |
55.54 |
59.86 |
4.32 |
| 7 |
Franciscus, Kimberly A (15)
|
MAUN-2 |
58.46 |
1:00.83 |
2.37 |
| 8 |
McGovern, Katelyn O (15)
|
MAGRA |
1:00.06 |
1:01.03 |
0.97 |
| 9 |
Koloseike, Claire E (15)
|
MALCY |
1:00.18 |
1:02.69 |
2.51 |
| 10 |
Reid, Alexandria C (17)
|
MAACST |
1:03.49 |
1:04.99 |
1.50 |
| 11 |
Burke, Alexandra R (15)
|
MAUN-3 |
1:07.08 |
1:11.46 |
4.38 |
|
Mullins, Kirsten A (17)
|
MAGRA |
|
NS |
- |
|
Breiner, Madison T (16)
|
MAGRA |
56.34 |
NS |
- |
|
Rochon, Riley T (17)
|
MACAA |
53.48 |
NS |
- |
|
Cleveland, Riley C (16)
|
MALCY |
1:03.53 |
NS |
- |
|
McDermott, Breanne (17)
|
MALCY |
57.76 |
NS |
- |
|
Pastris, Alexandra M (15)
|
MATRI |
1:10.99 |
NS |
- |
|
Franciscus, Samantha L (18)
|
MAUN-2 |
1:02.11 |
NS |
- |
|
May, Sophie N (17)
|
MAUN-4 |
53.61 |
NS |
- |