| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Mallari, Jewel (10)
|
MASEA |
43.88 |
43.88 |
0.00 |
| 2 |
Yushan, Rachel (10)
|
MAPVA |
47.51 |
47.15 |
-0.36 |
| 3 |
Radhakrishnan, Sahana N (10)
|
NJSVY |
49.96 |
47.52 |
-2.44 |
| 4 |
Tran, Anna (10)
|
MAPVA |
46.44 |
47.71 |
1.27 |
| 5 |
Bukowski, Karolina (10)
|
CTWYW |
48.21 |
48.75 |
0.54 |
| 6 |
Boles, Karly J (10)
|
MAGAAC |
49.25 |
49.82 |
0.57 |
| 7 |
Cheng, Sophie (10)
|
NJBWTD |
49.17 |
50.77 |
1.60 |
| 8 |
Napier, Alyssa (9)
|
MAWEST |
53.69 |
51.05 |
-2.64 |
| 9 |
Latham, Julia M (10)
|
MASEA |
50.43 |
51.26 |
0.83 |
| 10 |
Keim, Lilliana E (9)
|
MASEA |
50.18 |
51.30 |
1.12 |
| 11 |
Cooper, Olivia J (10)
|
MAPAY |
50.83 |
51.44 |
0.61 |
| 12 |
Treglia, Megan E (10)
|
MAJW |
44.66 |
52.22 |
7.56 |
| 13 |
Do, Kaitlynn (10)
|
MAPVA |
53.68 |
52.80 |
-0.88 |
| 14 |
Cappola, Alexandra G (10)
|
MASSC |
45.12 |
53.45 |
8.33 |
| 15 |
Seidman, Jordan K (10)
|
MAGAAC |
53.39 |
54.23 |
0.84 |
| 16 |
Marks, Riley L (9)
|
MAPAY |
47.13 |
56.28 |
9.15 |