| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Wang, Jake (10)
|
MASSC |
42.52 |
40.29 |
-2.23 |
| 2 |
Derivaux, Charles H (10)
|
MAJW |
45.13 |
42.58 |
-2.55 |
| 3 |
Rhee, Nehemiah Y (10)
|
NJSCAR |
44.17 |
43.10 |
-1.07 |
| 4 |
Broadbelt, Colin D (10)
|
MAJW |
41.41 |
46.89 |
5.48 |
| 5 |
Behm, John E (10)
|
MAJW |
40.21 |
47.01 |
6.80 |
| 6 |
Gravuer, Brody M (10)
|
MADST |
49.91 |
49.68 |
-0.23 |
| 7 |
Kim, Joshua (9)
|
MAJW |
44.47 |
49.71 |
5.24 |
| 8 |
Pittala, Sritan (10)
|
MADST |
51.08 |
50.04 |
-1.04 |
| 9 |
Pasquini, Alexander A (9)
|
MAJW |
45.47 |
51.04 |
5.57 |
| 10 |
Raue, Colin T (10)
|
MAJW |
44.12 |
51.66 |
7.54 |
| 11 |
Dec, Bartosz N (9)
|
MAJW |
48.38 |
53.17 |
4.79 |
| 12 |
Yurkewicz, Alex N (10)
|
MAJW |
47.19 |
53.62 |
6.43 |
| 13 |
Verbanas, Conner J (10)
|
MADST |
47.36 |
55.16 |
7.80 |
| 14 |
Toker-Katskee, Alexander P (9)
|
PVTOLL |
58.99 |
1:01.40 |
2.41 |
| 15 |
Blot, William J (9)
|
PVTOLL |
58.99 |
1:04.57 |
5.58 |
|
Murphy, Nolan W (10)
|
MASSC |
44.83 |
NS |
- |
|
Zhu, Kerry W (10)
|
PVTOLL |
58.99 |
NS |
- |