| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Ritter, Kassandra M (10)
|
MANEYS |
32.26 |
34.68 |
2.42 |
| 2 |
Cort, Julia E (9)
|
MALVA |
|
35.65 |
- |
| 3 |
Otero-Rehrig, Hazel C (10)
|
MAPAAC |
36.03 |
35.73 |
-0.30 |
| 4 |
Connell, Samantha R (10)
|
MALVA |
36.60 |
36.48 |
-0.12 |
| 5 |
Price, Katryna M (10)
|
MAEMAC |
38.48 |
38.80 |
0.32 |
| 6 |
Horvat, Isabella E (9)
|
MAUN-2 |
39.12 |
40.26 |
1.14 |
| 7 |
Ciaccia, Kayla J (10)
|
MALVA |
|
40.35 |
- |
| 8 |
Denton, Brielle M (10)
|
MAPSC |
|
42.47 |
- |
| 9 |
Harper, Jordyn C (9)
|
MAEMAC |
|
43.47 |
- |
| 10 |
Fastenberg, Christina N (9)
|
MAPAAC |
50.06 |
54.54 |
4.48 |
|
Pechulis, Emelia C (9)
|
MAEMAC |
38.81 |
NS |
- |