| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Ewer, Lily C (7)
|
MAMSA |
46.14 |
40.97 |
-5.17 |
| 2 |
LaFrazza-Schwartz, Cameron C (8)
|
MANLAC |
|
41.36 |
- |
| 3 |
Rittenhouse, Hailey P (8)
|
MAMSA |
|
42.74 |
- |
| 4 |
Ochs, Lia M (8)
|
MAUN-2 |
|
43.07 |
- |
| 5 |
May, Piper C (8)
|
MAMSA |
49.15 |
43.15 |
-6.00 |
| 6 |
Karasinski, Helen (7)
|
MAPENN |
51.04 |
45.92 |
-5.12 |
| 7 |
Trego, Natalie K (7)
|
MANLAC |
|
46.10 |
- |
| 8 |
Lukac, Cora G (8)
|
MANLAC |
|
48.06 |
- |
| 9 |
Dare, Reagan A (8)
|
MANLAC |
|
51.42 |
- |
| 10 |
Yocum, Jordyn A (6)
|
MANLAC |
|
57.71 |
- |
| 11 |
Babb, Constance G (5)
|
MANLAC |
|
1:03.52 |
- |
| 12 |
Rolfe, Brynn E (6)
|
MAMSA |
|
1:12.20 |
- |
| 13 |
Dare, Harper E (6)
|
MANLAC |
|
1:15.51 |
- |