| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Petrakis, Grace L (8)
|
MAUN-2 |
35.07 |
36.87 |
1.80 |
| 2 |
Lyons, Abigail C (8)
|
MAWAC |
42.13 |
38.44 |
-3.69 |
| 3 |
Lyons, Ella J (8)
|
MAWAC |
42.48 |
39.58 |
-2.90 |
| 4 |
Ashman, Aubrey V (8)
|
MAWAC |
44.50 |
40.91 |
-3.59 |
| 5 |
Persun, Kiley N (8)
|
MAWASC |
53.43 |
43.82 |
-9.61 |
| 6 |
Braun, Casey (7)
|
MAUDAC |
58.66 |
44.94 |
-13.72 |
| 7 |
Devlin, Mia L (8)
|
MAWAC |
|
45.59 |
- |
| 8 |
Bahn, Melanie C (8)
|
MATHY |
|
46.60 |
- |
| 9 |
El Ghissassi, Sidra S (7)
|
NIUN-7 |
44.58 |
46.88 |
2.30 |
| 10 |
Aiello, Taylor M (7)
|
MAWAC |
|
47.14 |
- |
| 11 |
Landers, Meghan E (7)
|
MATHY |
|
47.30 |
- |
| 12 |
Murray, Lily E (8)
|
MABDA |
50.63 |
47.72 |
-2.91 |
| 13 |
Geberth, Rebecca L (8)
|
MATHY |
|
49.47 |
- |
| 14 |
Blodgett, Anna J (8)
|
AMSASC |
51.53 |
49.49 |
-2.04 |
| 15 |
Gulli, Kendall J (7)
|
MAWAC |
|
50.92 |
- |
| 16 |
Orr, Aubrie E (8)
|
MAMARA |
|
52.50 |
- |
| 17 |
Krista, Lennon M (7)
|
MALAC |
52.86 |
54.39 |
1.53 |
| 18 |
Behun, Kathryn E (6)
|
MAUN-1 |
1:10.82 |
54.83 |
-15.99 |
| 19 |
Welgosh, Anika R (8)
|
MAUMAC |
|
55.09 |
- |
| 20 |
Persun, Kloey E (7)
|
MAWASC |
|
59.17 |
- |
| 21 |
Rivera, Sofia (6)
|
MAT85 |
|
59.95 |
- |
| 22 |
Boettger, Rhiley M (6)
|
MAMARA |
|
1:11.68 |
- |
|
Grohsman, Elizabeth M (7)
|
MAUDAC |
|
NS |
- |