| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
McKenna, Paige S (14)
|
MAPAAC |
5:19.51 |
5:24.50 |
4.99 |
| 2 |
Kuo, Jessica Y (13)
|
MACBST |
5:40.51 |
5:35.95 |
-4.56 |
| 3 |
Stark, Ilene R (13)
|
MACBST |
6:09.32 |
5:43.53 |
-25.79 |
| 4 |
Szerencsits, Olivia S (13)
|
MAPAAC |
5:55.73 |
5:43.84 |
-11.89 |
| 5 |
Fritz, Madison L (14)
|
MANPAC |
5:29.37 |
5:44.20 |
14.83 |
| 6 |
Bubser, Kelci L (14)
|
MAPAAC |
5:43.70 |
5:47.77 |
4.07 |
| 7 |
Ehrnfeldt, Megan E (13)
|
MABYNS |
6:36.65 |
5:48.43 |
-48.22 |
| 8 |
Fleischaker, Leyna S (13)
|
MAPAAC |
|
5:57.84 |
- |
| 9 |
Gravuer, Kiersten M (14)
|
MANRG |
6:07.98 |
6:00.86 |
-7.12 |
| 10 |
Lee, Lana E (14)
|
MAUDAC |
|
6:04.37 |
- |
| 11 |
Dolan, Rachel M (13)
|
MAPAAC |
|
6:15.75 |
- |
| 12 |
Walls, Lauren R (13)
|
MAPAAC |
6:29.37 |
6:16.83 |
-12.54 |
| 13 |
McCouch, Sandra L (13)
|
MADCAA |
6:23.01 |
6:17.51 |
-5.50 |
| 14 |
May, Georgia S (14)
|
MADYD |
|
6:20.66 |
- |
| 15 |
Sheridan, Isabel L (13)
|
MAPAAC |
6:40.06 |
6:34.09 |
-5.97 |
|
Persaud, Hannah (14)
|
MAPFY |
6:32.41 |
DQ |
- |