| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Chiu, Alexander (16)
|
MASJAC |
55.35 |
55.06 |
-0.29 |
| 2 |
Irwin, Robert W (15)
|
MASJAC |
|
58.99 |
- |
| 3 |
Hendricks, Branden P (18)
|
MAWAC |
55.96 |
59.01 |
3.05 |
| 4 |
Zeigler, Jack (16)
|
MASJAC |
57.55 |
59.79 |
2.24 |
| 5 |
Overton, Christian L (16)
|
MASJAC |
1:00.53 |
59.87 |
-0.66 |
| 6 |
Watts, Matthew J (17)
|
MASJAC |
|
1:00.40 |
- |
| 7 |
Sommeling, Michael (16)
|
MASJAC |
58.67 |
1:00.92 |
2.25 |
| 8 |
Seybold, Colin P (17)
|
MASJAC |
1:05.92 |
1:02.31 |
-3.61 |
| 9 |
Santarpio, James A (16)
|
MASJAC |
|
1:02.97 |
- |
| 10 |
Maier, Andrew D (15)
|
MASJAC |
1:02.98 |
1:04.17 |
1.19 |
| 11 |
Dadhania, Jay N (15)
|
MASJAC |
1:01.96 |
1:05.01 |
3.05 |
| 12 |
Bowen, Connor P (15)
|
MAUNSJ |
|
1:07.21 |
- |
| 13 |
Krieger, Aiden D (15)
|
MAWAC |
1:10.86 |
1:08.26 |
-2.60 |
| 14 |
Oleaga, Christian A (15)
|
MASJAC |
1:19.47 |
1:17.78 |
-1.69 |