| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Paglia, Sydney J (15)
|
MASSC |
56.72 |
1:00.84 |
4.12 |
| 2 |
Shema, Erin O (15)
|
MAUNSS |
57.06 |
1:01.85 |
4.79 |
| 3 |
Brandt, Mackenzie A (13)
|
MAUNSS |
1:01.08 |
1:02.28 |
1.20 |
| 4 |
Aguirre, Madeleine T (17)
|
MASSC |
58.31 |
1:05.01 |
6.70 |
| 5 |
Feuzeu Mekoue, Laurielle Kel (13)
|
MASSC |
1:01.77 |
1:05.39 |
3.62 |
| 6 |
Ott, Madeleine F (14)
|
MASSC |
1:05.27 |
1:08.95 |
3.68 |
| 7 |
Shifflett, Ella M (13)
|
MASSC |
1:09.21 |
1:10.45 |
1.24 |
| 8 |
Wilson, Mekhi S (13)
|
MASSC |
1:03.06 |
1:11.06 |
8.00 |
| 9 |
Slupianek, Helena (14)
|
MASSC |
1:07.98 |
1:11.28 |
3.30 |
| 10 |
Jones, Laura G (15)
|
MASSC |
1:11.13 |
1:13.20 |
2.07 |
| 11 |
Paglia, Sophia B (13)
|
MASSC |
|
1:14.03 |
- |
| 12 |
Scott, Ella G (13)
|
MASSC |
1:12.62 |
1:15.59 |
2.97 |
| 13 |
Jones, Alexandra S (15)
|
MASSC |
1:14.06 |
1:16.64 |
2.58 |
| 14 |
Mitchell, Julia G (14)
|
MASSC |
1:14.54 |
1:16.68 |
2.14 |
|
Hipp, Alexa R (16)
|
MASSC |
1:01.70 |
NS |
- |