| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
O'Brien, Makenzie M (10)
|
MDEST |
3:20.12 |
3:14.24 |
-5.88 |
| 2 |
Matanoski, Genevieve A (9)
|
MDEST |
3:23.15 |
3:24.68 |
1.53 |
| 3 |
Weker, Zoe M (10)
|
MDCATY |
3:03.04 |
3:29.66 |
26.62 |
| 4 |
Mackey, Allyson (Ally) C (10)
|
MDEST |
3:40.18 |
3:30.44 |
-9.74 |
| 5 |
Harvey, Shannon E (10)
|
MDNAAC |
3:32.25 |
3:31.61 |
-0.64 |
| 6 |
Pokharel, Suvali (10)
|
MDEST |
3:29.58 |
3:33.31 |
3.73 |
| 7 |
Smith, Madison E (10)
|
MDEST |
3:39.93 |
3:36.95 |
-2.98 |
| 8 |
Cowley, Jenna S (10)
|
MDEST |
3:45.24 |
3:38.42 |
-6.82 |
| 9 |
Gensley, Alyssa M (9)
|
MDROCK |
3:42.44 |
4:12.54 |
30.10 |
|
Elliott, Aaliyah A (9)
|
MDEST |
3:57.67 |
DQ |
- |
|
Svejda, Josephine M (10)
|
MDEST |
3:51.11 |
DQ |
- |