| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Aclao, Joromya (14)
|
MRQNS |
26.41 |
26.43 |
0.02 |
| 2 |
Conrad, Sabrina L (13)
|
MRRA |
26.05 |
26.51 |
0.46 |
| 2 |
Leal, Nancy (14)
|
MRRED |
26.42 |
26.51 |
0.09 |
| 4 |
Chan, Sarah E (14)
|
MRFLY |
26.35 |
26.97 |
0.62 |
| 5 |
Hyndman, Kailah F (14)
|
MRHES |
26.63 |
26.98 |
0.35 |
| 6 |
Lau, Anabelle (14)
|
MRQNS |
26.93 |
26.99 |
0.06 |
| 7 |
Ronaghan, Olivia R (13)
|
MRQNS |
26.99 |
27.08 |
0.09 |
| 8 |
Bice, Casey C (14)
|
MRQNS |
27.63 |
27.15 |
-0.48 |
| 9 |
LePique, Maya F (14)
|
MRRED |
27.09 |
27.44 |
0.35 |
| 10 |
Maffucci, Ava J (14)
|
MRRA |
28.58 |
27.50 |
-1.08 |
| 11 |
Knutson, Sophia (13)
|
MRHYB |
28.48 |
27.54 |
-0.94 |
| 12 |
Sandoval, Candela (14)
|
MRQNS |
27.97 |
27.70 |
-0.27 |
| 13 |
Conlan, Olivia (14)
|
MRQNS |
27.17 |
27.73 |
0.56 |
| 14 |
Kuduk, Norah (13)
|
MRRA |
28.16 |
28.02 |
-0.14 |
| 15 |
Liang, Victoria (14)
|
MRGATE |
28.50 |
28.04 |
-0.46 |
| 16 |
Wang, Laurie (14)
|
MRGATE |
28.60 |
28.73 |
0.13 |
| 17 |
Poschmann, Katie G (13)
|
MRHYB |
29.00 |
28.77 |
-0.23 |
| 18 |
Palmer, Avery N (13)
|
MRRED |
28.99 |
29.21 |
0.22 |
| 19 |
Kovacs, Isabella M (14)
|
MRQNS |
29.00 |
29.45 |
0.45 |
| 20 |
Marcotullio, Isabella X (13)
|
MRRED |
28.99 |
29.90 |
0.91 |
| 21 |
Battle, Jada J (13)
|
MRFLY |
30.22 |
29.95 |
-0.27 |
| 22 |
Chang, Chloe A (13)
|
MRFLY |
29.73 |
30.09 |
0.36 |
| 23 |
Porr, Nicole M (14)
|
MRFLY |
30.02 |
30.20 |
0.18 |
| 24 |
Lutchmansingh, Emily E (14)
|
MRRED |
28.99 |
30.39 |
1.40 |
| 25 |
Antman, Sabrina L (13)
|
MRRA |
28.36 |
30.82 |
2.46 |
| 26 |
Thrower, Nia S (13)
|
MRHES |
31.26 |
31.21 |
-0.05 |
| 27 |
Gillin, Valentina T (13)
|
MRFLY |
32.09 |
32.31 |
0.22 |
| 28 |
Venezia, Madelyn S (13)
|
MRRA |
33.39 |
32.39 |
-1.00 |
| 29 |
Reid, Vanessa N (13)
|
MRFLY |
33.06 |
34.34 |
1.28 |
| 30 |
Conner, Claire (14)
|
MRHYB |
29.00 |
37.36 |
8.36 |
|
Suteja, Kezia S (14)
|
MRNCAC |
28.00 |
NS |
- |
|
Lei, Anya (13)
|
MRUN |
28.17 |
NS |
- |