| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Pace, Isabelle B (8)
|
NCBAC |
46.45 |
43.61 |
-2.84 |
| 2 |
Hughes, Chloe A (8)
|
NCWOW |
46.39 |
45.61 |
-0.78 |
| 3 |
Crane, Taylor M (8)
|
NCWOW |
44.85 |
46.50 |
1.65 |
| 4 |
Phillips, Abbie E (8)
|
NCGFSC |
50.53 |
46.91 |
-3.62 |
| 5 |
Bollenbacher, Heidi F (8)
|
NCWOW |
57.62 |
47.41 |
-10.21 |
| 6 |
Ciancanelli, Isabella R (8)
|
NCGFSC |
49.84 |
47.61 |
-2.23 |
| 7 |
Caron, Tess A (8)
|
NCBAC |
52.93 |
49.66 |
-3.27 |
| 8 |
Uebbing, CoCo A (7)
|
NCWOW |
57.43 |
49.89 |
-7.54 |
| 9 |
Phillips, Sarah C (7)
|
NCWOW |
54.57 |
49.97 |
-4.60 |
| 10 |
Williams, Ava M (8)
|
NCBAC |
1:05.78 |
50.13 |
-15.65 |
| 11 |
Gordon, Kennedy S (8)
|
NCBAC |
55.10 |
51.35 |
-3.75 |
| 12 |
Derry, Violet M (8)
|
NCWOW |
54.87 |
54.62 |
-0.25 |
| 13 |
Pace, Olivia T (7)
|
NCBAC |
56.26 |
56.29 |
0.03 |
| 14 |
Boehmer, Opal G (7)
|
NCBAC |
1:00.16 |
56.40 |
-3.76 |
| 15 |
Gordon, Harper E (8)
|
NCBAC |
57.36 |
57.06 |
-0.30 |
| 16 |
Phillips, Kelly A (6)
|
NCWOW |
|
58.44 |
- |
| 17 |
Averitt, Skyleigh F (6)
|
SCMBR |
55.00 |
59.17 |
4.17 |
| 18 |
Long, Abigail G (8)
|
NCBAC |
1:03.25 |
1:00.18 |
-3.07 |
| 19 |
Eickhoff, Charlotte D (7)
|
NCGFSC |
1:10.19 |
1:00.25 |
-9.94 |
| 20 |
Morris, Caroline G (7)
|
NCBAC |
|
1:03.20 |
- |
| 21 |
Johnson, Eliza A (7)
|
NCWOW |
|
1:03.31 |
- |
| 22 |
Senter, Ainsley J (6)
|
NCBAC |
1:12.20 |
1:03.54 |
-8.66 |
| 23 |
Harrington, Jordan T (7)
|
NCWOW |
1:06.36 |
1:03.65 |
-2.71 |
| 24 |
McMullan, Blakely S (5)
|
NCBAC |
1:07.03 |
1:06.26 |
-0.77 |
| 25 |
Piepgrass, Anna E (7)
|
NCWOW |
|
1:08.16 |
- |
| 26 |
Charkoudian, Sarah (5)
|
NCCFAC |
|
1:35.52 |
- |
|
Sneed, Mylee N (6)
|
NCBAC |
|
DQ |
- |
|
Poorbaugh, Harper D (7)
|
NCGFSC |
1:17.78 |
NS |
- |