| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Crane, Taylor M (8)
|
NCWOW |
36.24 |
36.68 |
0.44 |
| 2 |
Pace, Isabelle B (8)
|
NCBAC |
37.63 |
38.47 |
0.84 |
| 3 |
Caron, Tess A (8)
|
NCBAC |
39.40 |
39.48 |
0.08 |
| 4 |
Hughes, Chloe A (8)
|
NCWOW |
40.44 |
40.60 |
0.16 |
| 5 |
Williams, Ava M (8)
|
NCBAC |
43.34 |
41.01 |
-2.33 |
| 6 |
Whiteside, Kayley M (8)
|
NCBAC |
45.43 |
41.94 |
-3.49 |
| 7 |
Phillips, Abbie E (8)
|
NCGFSC |
42.68 |
42.52 |
-0.16 |
| 8 |
Uebbing, CoCo A (7)
|
NCWOW |
44.45 |
42.74 |
-1.71 |
| 9 |
Bollenbacher, Heidi F (8)
|
NCWOW |
44.92 |
43.34 |
-1.58 |
| 10 |
Ciancanelli, Isabella R (8)
|
NCGFSC |
41.74 |
43.38 |
1.64 |
| 11 |
Volety, Amulya M (8)
|
NCWOW |
43.96 |
45.35 |
1.39 |
| 12 |
Jeeves, Grace H (7)
|
NCWOW |
43.77 |
46.55 |
2.78 |
| 13 |
Derry, Violet M (8)
|
NCWOW |
48.90 |
46.64 |
-2.26 |
| 14 |
Gordon, Harper E (8)
|
NCBAC |
56.95 |
49.08 |
-7.87 |
| 15 |
Zeron, Finley K (8)
|
NCBAC |
47.45 |
49.59 |
2.14 |
| 16 |
Gordon, Kennedy S (8)
|
NCBAC |
47.02 |
51.36 |
4.34 |
| 17 |
Pace, Olivia T (7)
|
NCBAC |
52.75 |
52.23 |
-0.52 |
| 18 |
Phillips, Kelly A (6)
|
NCWOW |
52.26 |
53.16 |
0.90 |
| 19 |
Long, Abigail G (8)
|
NCBAC |
1:00.70 |
53.41 |
-7.29 |
| 20 |
Mosley, Bleu (8)
|
NCBAC |
|
53.74 |
- |
| 21 |
Boehmer, Opal G (7)
|
NCBAC |
1:02.24 |
55.33 |
-6.91 |
| 22 |
Johnson, Eliza A (7)
|
NCWOW |
1:01.08 |
56.42 |
-4.66 |
| 23 |
Morris, Caroline G (7)
|
NCBAC |
1:12.06 |
57.24 |
-14.82 |
| 24 |
Eickhoff, Charlotte D (7)
|
NCGFSC |
1:01.59 |
57.34 |
-4.25 |
| 25 |
Senter, Ainsley J (6)
|
NCBAC |
1:01.23 |
59.04 |
-2.19 |
| 26 |
Harrington, Jordan T (7)
|
NCWOW |
1:12.09 |
1:07.30 |
-4.79 |
| 27 |
Bixby, Mia C (8)
|
NCWOW |
|
1:11.08 |
- |
| 28 |
House, Asheford R (6)
|
NCCFAC |
1:10.29 |
1:14.24 |
3.95 |
| 29 |
McMullan, Blakely S (5)
|
NCBAC |
1:19.05 |
1:16.56 |
-2.49 |
| 30 |
Charkoudian, Sarah (5)
|
NCCFAC |
|
1:52.94 |
- |
|
Berg, Chloe (8)
|
NCCFAC |
|
DQ |
- |
|
Piepgrass, Anna E (7)
|
NCWOW |
|
NS |
- |