| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
McRea, Micah W (16)
|
NCCVAC |
47.72 |
49.45 |
1.73 |
| 2 |
Leatherman, Brady H (16)
|
NCCVAC |
47.27 |
50.69 |
3.42 |
| 3 |
Martin, Micah R (16)
|
NCCVAC |
51.71 |
51.44 |
-0.27 |
| 4 |
Morris, Nicholas K (17)
|
NCSAC |
49.80 |
52.12 |
2.32 |
| 5 |
Carroll, Brody W (15)
|
NCCVAC |
51.96 |
52.30 |
0.34 |
| 6 |
Little III, Thomas W (16)
|
NCCVAC |
51.49 |
52.98 |
1.49 |
| 7 |
Miller, Nolan M (15)
|
NCMSC |
52.74 |
53.49 |
0.75 |
| 8 |
Guerriero, Nicholas D (17)
|
NCSAC |
51.37 |
54.07 |
2.70 |
| 9 |
Annas, Andrew S (17)
|
NCLENR |
51.58 |
54.71 |
3.13 |
| 10 |
Sacchetti, Mario T (16)
|
NCMSC |
53.08 |
54.78 |
1.70 |
| 11 |
Negrete-Abreu, Israel (16)
|
NCCVAC |
55.73 |
56.79 |
1.06 |
| 12 |
McKee, Garrett S (16)
|
NCWA |
52.35 |
57.11 |
4.76 |
| 13 |
Vandergrift, Joshua (17)
|
NCWA |
58.11 |
58.52 |
0.41 |
| 14 |
Cooper, Christian B (18)
|
NCWA |
1:00.91 |
1:02.24 |
1.33 |
| 15 |
Yelton, Hank G (17)
|
NCMSC |
1:07.40 |
1:05.05 |
-2.35 |
| 16 |
Messenger, Dalton A (12)
|
NCSAC |
1:07.64 |
1:07.53 |
-0.11 |
| 17 |
Dye, Andrew S (15)
|
NCLENR |
1:27.95 |
1:24.19 |
-3.76 |
| 18 |
Williams, Jaasiel L (11)
|
NCGG |
|
1:43.72 |
- |
|
Hinson, Paxton T (15)
|
NCGG |
1:12.16 |
NS |
- |
|
Williams, Brandon L (17)
|
NCWA |
1:01.25 |
NS |
- |