| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Sic, Ana N (8)
|
NCMSC |
24.77 |
22.26 |
-2.51 |
| 2 |
Farris, Gracie L (8)
|
NCMSC |
25.69 |
24.12 |
-1.57 |
| 3 |
Handy, Elsie R (8)
|
NCCVAC |
23.99 |
24.44 |
0.45 |
| 4 |
Jones, Paige B (7)
|
NCGG |
28.38 |
25.24 |
-3.14 |
| 5 |
Vandergrift, Katelyn E (7)
|
NCWA |
24.95 |
25.63 |
0.68 |
| 6 |
Walters, Liberty J (8)
|
NCCVAC |
27.08 |
27.99 |
0.91 |
| 7 |
Johnson, Valerie D (6)
|
NCCVAC |
31.84 |
29.70 |
-2.14 |
| 8 |
Griffith, Isabella M (8)
|
NCLENR |
28.81 |
30.06 |
1.25 |
| 9 |
Millsaps, Katherine G (5)
|
NCCVAC |
33.82 |
30.61 |
-3.21 |
| 10 |
Stawecki, Riley A (7)
|
NCLENR |
31.27 |
31.15 |
-0.12 |
| 11 |
Hollar, Cambrie R (6)
|
NCCVAC |
|
32.24 |
- |
| 12 |
Smith, Jayden N (7)
|
NCLENR |
34.93 |
34.97 |
0.04 |
| 13 |
Beltran-Trejo, Fernanda (6)
|
NCCVAC |
39.98 |
40.46 |
0.48 |