| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Thornburg, Emma G (12)
|
NCGG |
42.62 |
36.21 |
-6.41 |
| 2 |
Barrick, Lauren T (11)
|
NCSAC |
37.75 |
36.31 |
-1.44 |
| 3 |
Caudle, Riley S (11)
|
NCCVAC |
39.36 |
40.43 |
1.07 |
| 4 |
Murphy, Riley J (12)
|
NCLENR |
46.25 |
41.86 |
-4.39 |
| 5 |
Olguin-Abreu, Denise (12)
|
NCCVAC |
42.03 |
41.96 |
-0.07 |
| 6 |
Beltran-Trejo, Nicole (12)
|
NCCVAC |
41.21 |
42.10 |
0.89 |
| 7 |
Rodriquez, Maria V (11)
|
NCMSC |
40.80 |
42.57 |
1.77 |
| 8 |
Olvera, Karina (12)
|
NCCVAC |
43.60 |
42.84 |
-0.76 |
| 9 |
Kale, Zelina M (11)
|
NCCVAC |
52.18 |
49.71 |
-2.47 |
| 10 |
Yelton, Lucy E (11)
|
NCMSC |
52.42 |
49.97 |
-2.45 |
|
Walters, Abigail R (11)
|
NCCVAC |
1:01.70 |
NS |
- |