| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Simpson, Jake R (11)
|
NCGG |
34.23 |
34.81 |
0.58 |
| 2 |
Stillwagon, Matthew H (12)
|
NCNMA |
34.35 |
35.36 |
1.01 |
| 3 |
Major, Zachary A (12)
|
NCGG |
38.11 |
40.12 |
2.01 |
| 4 |
Stoliker III, Dornford W (12)
|
NCGG |
40.89 |
40.40 |
-0.49 |
| 5 |
Miller, Emerson M (11)
|
NCMSC |
43.07 |
41.01 |
-2.06 |
| 6 |
Cox, John H (12)
|
NCAQTC |
|
41.14 |
- |
| 7 |
Rhodes, Caleb E (11)
|
NCAQTC |
46.95 |
43.33 |
-3.62 |
| 8 |
Hareyan, Harutiun (12)
|
NCCVAC |
|
48.09 |
- |
| 9 |
Henage, Teague G (12)
|
NCWA |
48.90 |
49.55 |
0.65 |
| 10 |
Ruebusch, Connor C (11)
|
NCCVAC |
52.26 |
55.69 |
3.43 |
|
Castellanos-Morales, Sebasti (11)
|
NCCVAC |
48.53 |
NS |
- |