| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Riley, Lilly A (10)
|
NCGG |
39.20 |
40.17 |
0.97 |
| 2 |
Mungro-Turowski, Madison L (10)
|
NCMSC |
42.01 |
43.26 |
1.25 |
| 3 |
Fayed, Lana C (10)
|
NCGG |
42.84 |
44.38 |
1.54 |
| 4 |
Kohler, Madison N (9)
|
NCWA |
44.65 |
45.02 |
0.37 |
| 5 |
Kelley, Aubrey L (10)
|
NCNMA |
42.50 |
45.63 |
3.13 |
| 6 |
Lopez, Marta B (10)
|
NCMSC |
50.26 |
46.26 |
-4.00 |
| 7 |
Rodriquez, Maria V (10)
|
NCMSC |
|
48.60 |
- |
| 8 |
Lawrence, Amelia S (10)
|
NCWA |
55.21 |
54.43 |
-0.78 |
| 9 |
Negrete-Perez, Stephanie (10)
|
NCCVAC |
1:03.60 |
59.39 |
-4.21 |
| 10 |
Ledbetter, Nevaeh R (10)
|
NCNMA |
|
1:05.43 |
- |
| 11 |
Ashley, Riley A (9)
|
NCNMA |
1:18.60 |
1:28.80 |
10.20 |
|
Jones, Courtney A (9)
|
NCNMA |
1:12.87 |
DQ |
- |
|
Wilborn, Margaret M (9)
|
NCAQTC |
|
NS |
- |