| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Rose, Sydney S (12)
|
NCCVAC |
32.24 |
32.28 |
0.04 |
| 2 |
Yercheck, Gabrielle E (12)
|
NCWA |
38.01 |
32.49 |
-5.52 |
| 3 |
McNeal, Mila G (12)
|
NCGG |
32.16 |
33.35 |
1.19 |
| 4 |
Connelly, Cassandra M (12)
|
NCNMA |
35.04 |
35.28 |
0.24 |
| 5 |
Outlaw, Lizzie L (12)
|
NCWA |
37.51 |
36.91 |
-0.60 |
| 6 |
Clontz, Leah J (12)
|
NCCVAC |
38.68 |
37.33 |
-1.35 |
| 7 |
Aragon-Zamora, Angelica (12)
|
NCCVAC |
43.85 |
38.79 |
-5.06 |
| 8 |
Huffman, Sydney M (12)
|
NCCVAC |
41.83 |
39.24 |
-2.59 |
| 9 |
Caudle, Riley S (11)
|
NCCVAC |
42.74 |
41.33 |
-1.41 |
| 10 |
Presley, Amelia B (11)
|
NCAQTC |
|
41.62 |
- |
| 11 |
Lynch, Kirsten N (11)
|
NCGG |
42.26 |
42.44 |
0.18 |
| 12 |
Olguin-Abreu, Denise (11)
|
NCCVAC |
42.03 |
44.03 |
2.00 |
| 13 |
Huffstickler, Anna E (12)
|
NCGG |
40.67 |
44.07 |
3.40 |
| 14 |
Lopez-Rodriquez, Naydelin D (11)
|
NCMSC |
46.23 |
44.25 |
-1.98 |
| 15 |
Connelly, Brooke A (11)
|
NCNMA |
40.46 |
44.59 |
4.13 |
| 16 |
Williams, Christabella (12)
|
NCAQTC |
45.00 |
44.89 |
-0.11 |
| 17 |
Wall, Olivia A (11)
|
NCWA |
48.33 |
50.02 |
1.69 |
| 18 |
Kale, Zelina M (11)
|
NCCVAC |
56.19 |
52.88 |
-3.31 |
| 19 |
Washburn, Aly A (12)
|
NCRCST |
|
1:09.62 |
- |
|
Harte, Jessica A (12)
|
NCGG |
|
DQ |
- |
|
Hilderbran, Ella N (11)
|
NCGG |
42.82 |
NS |
- |
|
Sacchetti, Isabella M (12)
|
NCMSC |
34.77 |
NS |
- |
|
Gundala, Dhanya S (12)
|
NCNMA |
53.26 |
NS |
- |