| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Snyder, Rachel A (16)
|
NCLOY |
29.93 |
28.95 |
-0.98 |
| 2 |
Johnson, Caroline G (16)
|
NCLOY |
32.40 |
31.29 |
-1.11 |
| 3 |
Claudy, Madison A (15)
|
NCUN |
35.95 |
31.88 |
-4.07 |
| 4 |
Fortin, Nicole E (17)
|
NCLOY |
32.74 |
31.94 |
-0.80 |
| 5 |
Filiault, Sophia K (16)
|
NCLOY |
33.36 |
31.99 |
-1.37 |
| 6 |
Ratcliff, Charlotte A (15)
|
NCLOY |
33.69 |
32.13 |
-1.56 |
| 7 |
Kluepfel, Valerie J (15)
|
NCUN |
35.02 |
32.66 |
-2.36 |
| 8 |
Rasbornik, Kensley K (17)
|
NCLOY |
30.83 |
32.75 |
1.92 |
| 9 |
Breeden, Abbey (16)
|
NCLOY |
37.98 |
36.89 |
-1.09 |
| 10 |
Aldridge, Olivia A (15)
|
NCLOY |
41.00 |
38.86 |
-2.14 |
|
Misener, Jacqueline M (16)
|
NCLOY |
29.70 |
NS |
- |