| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Ross, Christina N (14)
|
NCLOY |
33.62 |
35.04 |
1.42 |
| 2 |
Tidwell, Katherine E (13)
|
NCLOY |
36.48 |
36.92 |
0.44 |
| 3 |
Sursi, Madelyn E (14)
|
NCLOY |
36.39 |
37.61 |
1.22 |
| 4 |
Garlick, Macyn E (14)
|
NCLOY |
45.55 |
37.65 |
-7.90 |
| 5 |
Alker, Brianna M (14)
|
NCGCY |
|
38.85 |
- |
| 6 |
Respess, Josephine G (13)
|
NCLOY |
39.95 |
39.76 |
-0.19 |
| 7 |
Ferguson, Ashley M (13)
|
NCLOY |
41.15 |
40.34 |
-0.81 |
| 8 |
Bankirer, Ellery W (13)
|
NCLOY |
39.90 |
40.83 |
0.93 |
| 9 |
Torres, Saida F (13)
|
NCLOY |
43.29 |
43.02 |
-0.27 |
| 10 |
Buttar, Aliya K (14)
|
NCLOY |
41.08 |
45.52 |
4.44 |
| 11 |
Roussel, Line C (13)
|
NCLOY |
|
49.91 |
- |
|
Misener, Katrin J (13)
|
NCLOY |
35.13 |
NS |
- |