| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Bellissimo, Sophia G (10)
|
NCLOY |
29.30 |
29.95 |
0.65 |
| 2 |
Betancourt, Amelia H (10)
|
NCLOY |
30.72 |
31.53 |
0.81 |
| 3 |
Claborn, Alyssa A (10)
|
NCLOY |
31.40 |
31.88 |
0.48 |
| 4 |
McDonough, Addyson R (10)
|
NCLOY |
33.60 |
34.77 |
1.17 |
| 5 |
Tars, Meribel E (10)
|
NCGCY |
35.58 |
35.22 |
-0.36 |
| 6 |
Wiechers, Abigail F (10)
|
NCLOY |
|
37.38 |
- |
| 7 |
Doyle, Savannah M (10)
|
NCGCY |
43.22 |
37.49 |
-5.73 |
| 8 |
Filiault, Julia K (10)
|
NCLOY |
38.46 |
37.88 |
-0.58 |
| 9 |
Lawrence, Amelia S (10)
|
NCWA |
36.70 |
38.94 |
2.24 |
| 10 |
Carpenter, Addison L (10)
|
NCLOY |
|
39.60 |
- |
| 11 |
McGuire, Molly S (10)
|
NCLOY |
|
42.77 |
- |
| 12 |
Moyer, Rachel J (10)
|
NCXA |
43.15 |
44.01 |
0.86 |
| 13 |
Allen, Grace M (10)
|
NCLOY |
|
44.75 |
- |
| 14 |
Dingman, Kasey S (9)
|
NCGCY |
43.88 |
45.00 |
1.12 |
| 15 |
Hoots, Olivia L (10)
|
NCGCY |
|
45.06 |
- |
| 16 |
Waters, Londyn S (9)
|
NCGCY |
47.05 |
46.80 |
-0.25 |
| 17 |
Grubb, Emma M (9)
|
NCGCY |
53.16 |
47.03 |
-6.13 |
|
Misener, Leah J (10)
|
NCLOY |
33.11 |
NS |
- |