| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Ross, Christina N (14)
|
NCLOY |
26.21 |
26.87 |
0.66 |
| 2 |
Alker, Brianna M (14)
|
NCGCY |
26.61 |
27.05 |
0.44 |
| 3 |
Garlick, Macyn E (14)
|
NCLOY |
26.92 |
27.77 |
0.85 |
| 4 |
Sursi, Madelyn E (14)
|
NCLOY |
26.57 |
27.81 |
1.24 |
| 5 |
Tidwell, Katherine E (13)
|
NCLOY |
28.45 |
28.06 |
-0.39 |
| 6 |
Respess, Josephine G (13)
|
NCLOY |
28.12 |
29.37 |
1.25 |
| 7 |
Torres, Saida F (13)
|
NCLOY |
29.58 |
29.65 |
0.07 |
| 8 |
Bankirer, Ellery W (13)
|
NCLOY |
29.84 |
29.77 |
-0.07 |
| 9 |
Buttar, Aliya K (14)
|
NCLOY |
28.28 |
31.67 |
3.39 |
| 10 |
Roussel, Line C (13)
|
NCLOY |
|
32.42 |
- |
| 11 |
Ferguson, Ashley M (13)
|
NCLOY |
33.76 |
33.06 |
-0.70 |
|
Misener, Katrin J (13)
|
NCLOY |
27.96 |
NS |
- |