| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Burton, Piper I (15)
|
NCGCY |
30.75 |
31.09 |
0.34 |
| 2 |
Horton, Haley L (18)
|
NCGCY |
31.06 |
31.38 |
0.32 |
| 3 |
Snyder, Rachel A (15)
|
NCLOY |
35.23 |
32.62 |
-2.61 |
| 4 |
Misener, Riley M (18)
|
NCLOY |
34.00 |
33.29 |
-0.71 |
| 5 |
Folstad, Makenzie L (17)
|
NCLOY |
32.94 |
33.77 |
0.83 |
| 6 |
McDonough, Leah K (16)
|
NCLOY |
|
35.68 |
- |
| 7 |
Fortin, Nicole E (17)
|
NCLOY |
38.55 |
35.90 |
-2.65 |
| 8 |
Ratcliff, Charlotte A (15)
|
NCLOY |
41.87 |
35.98 |
-5.89 |
| 9 |
Hartwick, Olivia J (15)
|
NCGCY |
36.06 |
35.99 |
-0.07 |
| 10 |
Peoples, Aubrey N (15)
|
NCGCY |
33.93 |
36.06 |
2.13 |
| 11 |
Curtis, Audrey R (18)
|
NCGCY |
33.19 |
36.10 |
2.91 |
| 12 |
Joyce, Alaina M (17)
|
NCGCY |
34.93 |
36.99 |
2.06 |
| 13 |
Busse, Megan D (18)
|
NCLOY |
58.20 |
37.41 |
-20.79 |
| 14 |
Filiault, Sophia K (15)
|
NCLOY |
37.57 |
37.51 |
-0.06 |
| 15 |
Morris, Celeste A (15)
|
NCGCY |
35.91 |
37.61 |
1.70 |
| 16 |
Rasbornik, Kensley K (16)
|
NCLOY |
34.07 |
38.53 |
4.46 |
| 17 |
Folstad, Madison A (16)
|
NCLOY |
36.35 |
38.81 |
2.46 |
| 18 |
Vancil, Anna J (15)
|
NCGCY |
46.65 |
40.41 |
-6.24 |
| 19 |
Sorjonen, Taika A (15)
|
NCGCY |
|
42.12 |
- |
| 20 |
Aldridge, Olivia A (15)
|
NCLOY |
|
44.31 |
- |