| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Gatling, Ladaniel (13)
|
NCGCY |
29.42 |
28.66 |
-0.76 |
| 2 |
Womble, Ethan V (14)
|
NCGCY |
29.99 |
28.83 |
-1.16 |
| 3 |
Yeh, Ryan T (14)
|
NCLOY |
30.37 |
29.09 |
-1.28 |
| 4 |
McDonald, Benjamin M (14)
|
NCGCY |
28.53 |
29.17 |
0.64 |
| 5 |
Crawford, Payton S (14)
|
NCLOY |
|
29.26 |
- |
| 6 |
Carsten, Nathaniel D (14)
|
NCLOY |
30.38 |
29.62 |
-0.76 |
| 7 |
Esposito, William P (14)
|
NCGCY |
31.72 |
30.91 |
-0.81 |
| 8 |
Wachendorfer, Matthew P (13)
|
NCGCY |
32.84 |
30.98 |
-1.86 |
| 9 |
Rasbornik, Duncan J (14)
|
NCLOY |
31.47 |
31.12 |
-0.35 |
| 10 |
Zhang, Ray (13)
|
NCGCY |
31.14 |
31.71 |
0.57 |
| 11 |
Roling Stella, Vicenzo (14)
|
NCGCY |
|
32.15 |
- |
| 12 |
Miroshnichenko, Alexander A (14)
|
NCGCY |
31.43 |
32.22 |
0.79 |
| 13 |
Brewer, Ethan J (14)
|
NCLOY |
33.40 |
32.55 |
-0.85 |
| 14 |
Claborn, Nathan M (13)
|
NCLOY |
34.48 |
32.91 |
-1.57 |
| 16 |
Adams, Brady C (13)
|
NCLOY |
38.57 |
33.78 |
-4.79 |