| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Shaw, Warner A (10)
|
SCSWIM |
3:19.29 |
3:07.81 |
-11.48 |
| 2 |
Yang, Angela H (10)
|
NCYOTA |
3:08.68 |
3:08.14 |
-0.54 |
| 3 |
Salazar, Emilie Julianna (10)
|
VALY |
3:23.67 |
3:16.61 |
-7.06 |
| 4 |
Teague, Estelle M (10)
|
NCSTAR |
3:24.00 |
3:21.01 |
-2.99 |
| 5 |
Perry, Sophie E (10)
|
NCYOTA |
3:02.99 |
3:22.49 |
19.50 |
| 6 |
Roberts, Lauren K (10)
|
NCYOTA |
3:17.10 |
3:22.96 |
5.86 |
| 7 |
Mihaly, Chloe C (10)
|
NCCAT |
3:23.40 |
3:29.72 |
6.32 |
| 8 |
Walsh, Ayden E (10)
|
NCSGSA |
3:23.12 |
3:37.23 |
14.11 |
| 9 |
Roberts, Grace S (9)
|
NCCAT |
3:54.05 |
3:39.97 |
-14.08 |
| 10 |
Teague, Avalon G (8)
|
NCSTAR |
3:54.85 |
3:43.59 |
-11.26 |
| 11 |
Schonwalder, Olivia J (9)
|
NCCAT |
4:05.66 |
4:07.91 |
2.25 |
| 12 |
Ciancanelli, Isabella R (8)
|
NCGFSC |
4:55.11 |
4:21.88 |
-33.23 |
| 13 |
Stevens, Katherine S (7)
|
NCSTAR |
4:34.40 |
4:33.70 |
-0.70 |
|
Brown, Nya J (10)
|
SCSWIM |
3:19.62 |
DQ |
- |
|
Bartlett, Kyla E (9)
|
NCWOW |
|
DQ |
- |
|
Spreitzer, Aubree K (10)
|
NCCAT |
4:15.99 |
NS |
- |
|
Deveaux, Kennedy A (10)
|
SCSWIM |
|
NS |
- |