| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Howards, Nina P (9)
|
NCEAC |
46.31 |
34.09 |
-12.22 |
| 2 |
Roberts, Grace S (9)
|
NCCAT |
37.74 |
35.49 |
-2.25 |
| 3 |
Frank, Lillian M (9)
|
NCCAT |
36.51 |
37.46 |
0.95 |
| 4 |
Cain, Amelia S (9)
|
NCPAST |
38.36 |
38.85 |
0.49 |
| 5 |
Doyle, Eva (9)
|
NCHPSC |
41.00 |
39.06 |
-1.94 |
| 6 |
Silinski, Abigail A (9)
|
NCCAT |
39.85 |
39.49 |
-0.36 |
| 7 |
Schonwalder, Olivia J (9)
|
NCCAT |
40.73 |
40.10 |
-0.63 |
| 8 |
Schilling, Abigail R (9)
|
NCSGSA |
39.74 |
40.38 |
0.64 |
| 9 |
Brinker, Abigail H (9)
|
NCNUMA |
38.31 |
40.76 |
2.45 |
| 10 |
Coleman, Rori K (9)
|
NCPAST |
48.65 |
44.85 |
-3.80 |
| 11 |
Collins, Taylor E (9)
|
NCHPSC |
50.18 |
48.17 |
-2.01 |
| 12 |
Salamh, Kalea L (9)
|
NCCAT |
51.15 |
49.95 |
-1.20 |
| 13 |
Ruelas Reyes, Samantha (9)
|
NCHPSC |
46.91 |
50.01 |
3.10 |
| 14 |
Grove, Kaye T (9)
|
NCSGSA |
50.71 |
51.24 |
0.53 |
|
Morrow, Amelia G (9)
|
NCEAC |
32.16 |
NS |
- |
|
Britt, Olivia P (9)
|
NCNSS |
39.45 |
NS |
- |
|
Romeiser, Maren A (9)
|
NCCAT |
|
NS |
- |
|
Palmer, Caroline L (9)
|
NCCAT |
41.44 |
NS |
- |
|
Issatayeva, Nurilya K (9)
|
NCCAT |
|
NS |
- |