| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Cain, Amelia S (9)
|
NCPAST |
49.71 |
47.91 |
-1.80 |
| 2 |
Roberts, Grace S (9)
|
NCCAT |
53.99 |
49.01 |
-4.98 |
| 3 |
Najera, Aileen F (9)
|
NCHPSC |
54.23 |
49.69 |
-4.54 |
| 4 |
Howards, Nina P (9)
|
NCEAC |
50.22 |
49.80 |
-0.42 |
| 5 |
Silinski, Abigail A (9)
|
NCCAT |
51.36 |
49.88 |
-1.48 |
| 6 |
Schonwalder, Olivia J (9)
|
NCCAT |
52.75 |
50.35 |
-2.40 |
| 7 |
Britt, Olivia P (9)
|
NCNSS |
49.71 |
50.50 |
0.79 |
| 8 |
Frank, Lillian M (9)
|
NCCAT |
53.35 |
51.18 |
-2.17 |
| 9 |
Schilling, Abigail R (9)
|
NCSGSA |
53.42 |
53.85 |
0.43 |
| 10 |
Doyle, Eva (9)
|
NCHPSC |
58.95 |
54.54 |
-4.41 |
| 11 |
Brinker, Abigail H (9)
|
NCNUMA |
1:00.29 |
56.96 |
-3.33 |
| 12 |
Salamh, Kalea L (9)
|
NCCAT |
1:01.14 |
57.06 |
-4.08 |
| 13 |
Day, Lauren E (9)
|
NCHPSC |
56.14 |
57.90 |
1.76 |
| 14 |
Collins, Taylor E (9)
|
NCHPSC |
1:01.01 |
1:00.86 |
-0.15 |
| 15 |
Coleman, Rori K (9)
|
NCPAST |
1:14.93 |
1:12.01 |
-2.92 |
| 16 |
Grove, Kaye T (9)
|
NCSGSA |
1:15.47 |
1:14.62 |
-0.85 |
|
Romeiser, Maren A (9)
|
NCCAT |
|
NS |
- |
|
Palmer, Caroline L (9)
|
NCCAT |
1:07.07 |
NS |
- |
|
Issatayeva, Nurilya K (9)
|
NCCAT |
|
NS |
- |