| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Tidwell, Katherine E (12)
|
NCLOY |
43.85 |
40.12 |
-3.73 |
| 2 |
Overton, Grace A (12)
|
NCRSA |
42.98 |
42.81 |
-0.17 |
| 3 |
Riley, Erin N (11)
|
NCRSA |
44.54 |
43.05 |
-1.49 |
| 4 |
Bunger, Valerie R (11)
|
NCRSA |
50.76 |
44.75 |
-6.01 |
| 5 |
Bush, Savanna N (11)
|
NCLOY |
52.49 |
45.48 |
-7.01 |
| 6 |
Lewis, Lauren E (11)
|
NCTYDE |
47.76 |
45.59 |
-2.17 |
| 7 |
Respess, Josephine G (12)
|
NCLOY |
45.90 |
46.61 |
0.71 |
| 8 |
Abbott, Ella M (12)
|
NCHPSC |
47.31 |
46.81 |
-0.50 |
| 9 |
Rydzewski, Samantha G (11)
|
NCRSA |
52.44 |
47.59 |
-4.85 |
| 10 |
Pegram, Elizabeth C (12)
|
NCRSA |
48.99 |
47.66 |
-1.33 |
| 11 |
Brechko, Elena D (12)
|
NCTYDE |
|
49.19 |
- |
| 12 |
Nichols, Cassandra L (11)
|
NCRSA |
48.92 |
50.58 |
1.66 |
| 13 |
Yeh, Sabrina T (11)
|
NCLOY |
49.58 |
52.49 |
2.91 |
| 14 |
Dingle, Caidyn A (12)
|
NCHPSC |
51.95 |
52.62 |
0.67 |
| 15 |
Schuster, Ava P (12)
|
NCLOY |
51.63 |
53.91 |
2.28 |
| 16 |
Maxey, Mallory E (11)
|
NCTYDE |
|
55.09 |
- |
| 17 |
Gayford, Ellen B (11)
|
NCLOY |
56.37 |
56.15 |
-0.22 |
| 18 |
Steele, Ava C (11)
|
NCTYDE |
|
59.54 |
- |
| 19 |
Bullard, Kara G (11)
|
NCHPSC |
|
1:06.92 |
- |
|
Post, Alejandra M (11)
|
NCTYDE |
|
NS |
- |
|
Holgate, Emma S (12)
|
NCRSA |
52.18 |
NS |
- |