| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Hodge, Anna M (10)
|
VACCA |
2:41.32 |
2:36.99 |
-4.33 |
| 2 |
Teague, Estelle M (10)
|
NCSTAR |
2:42.30 |
2:37.56 |
-4.74 |
| 3 |
Carter, Maddi G (10)
|
VACCA |
3:08.45 |
2:41.97 |
-26.48 |
| 4 |
Byrnes, Jia L (10)
|
VACCA |
2:55.88 |
2:44.86 |
-11.02 |
| 5 |
Votta, Emma B (10)
|
VACCA |
3:06.30 |
2:50.91 |
-15.39 |
| 6 |
Carter, Emmi D (9)
|
VACCA |
3:24.57 |
2:52.66 |
-31.91 |
| 7 |
Kreth, Rachel O (10)
|
NCTEAM |
3:01.29 |
2:54.11 |
-7.18 |
| 8 |
Viar, Annaliese R (10)
|
VACCA |
3:14.11 |
2:55.81 |
-18.30 |
| 9 |
Ferreira, Izabella C (10)
|
NCTEAM |
|
2:57.74 |
- |
| 10 |
Overstreet, Layla R (9)
|
VACCA |
|
2:59.39 |
- |
| 11 |
Miller, Catherine F (10)
|
NCHPSC |
3:41.18 |
3:02.12 |
-39.06 |
| 12 |
Doyle, Eva (10)
|
NCHPSC |
|
3:15.48 |
- |
| 13 |
Moore, Caitlyn M (9)
|
NCASTF |
|
3:17.06 |
- |
| 15 |
Spurlock, Avery M (9)
|
VACCA |
|
3:22.20 |
- |
| 16 |
Ruelas Reyes, Samantha (10)
|
NCHPSC |
3:58.44 |
3:27.34 |
-31.10 |