| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Coates, Nina A (10)
|
NCSSS |
32.76 |
32.28 |
-0.48 |
| 2 |
York, Victoria T (10)
|
NCSSS |
36.69 |
36.29 |
-0.40 |
| 3 |
Stayskal, Addisyn L (9)
|
NCSSS |
37.83 |
37.52 |
-0.31 |
| 4 |
Najera, Lindsay L (6)
|
NCHPSC |
42.90 |
42.09 |
-0.81 |
| 5 |
Freigo, Brianna M (10)
|
NCSSS |
46.27 |
43.83 |
-2.44 |
| 6 |
Flanagan, Madeleine G (10)
|
NCSSS |
44.19 |
44.83 |
0.64 |
| 7 |
Miller, Catherine F (10)
|
NCHPSC |
48.59 |
45.03 |
-3.56 |
| 8 |
Kaczorowski, Veronica A (9)
|
NCSSS |
|
45.88 |
- |
| 9 |
Ruelas Reyes, Samantha (9)
|
NCHPSC |
55.84 |
46.91 |
-8.93 |
| 10 |
Creel, Kinsley E (8)
|
NCSSS |
|
49.40 |
- |
| 11 |
Jones, Emery (8)
|
NCHPSC |
|
50.16 |
- |
| 12 |
Heilman, Annabelle R (9)
|
NCSSS |
58.34 |
51.93 |
-6.41 |
| 13 |
Simon, Natalie (8)
|
NCHPSC |
|
1:03.57 |
- |
| 14 |
Hughes, Sophia A (7)
|
NCSSS |
1:12.39 |
1:06.36 |
-6.03 |
| 15 |
White, Cara G (6)
|
NCSSS |
|
1:34.04 |
- |