| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Hertweck, Graham E (18)
|
NCSTAR |
45.64 |
48.27 |
2.63 |
| 2 |
Nannucci, Pietro (17)
|
NCSGSA |
47.28 |
49.04 |
1.76 |
| 3 |
Svane-From, Thomas A (17)
|
NCMAC |
47.40 |
49.14 |
1.74 |
| 4 |
Graham, Payton L (17)
|
NCMAC |
47.89 |
50.04 |
2.15 |
| 5 |
Hollett, Andrew G (16)
|
NCMOR |
48.81 |
50.36 |
1.55 |
| 6 |
Tuning, Jeffrey J (15)
|
NCWAVE |
49.19 |
50.44 |
1.25 |
| 7 |
Keaney, Reilly E (14)
|
NCSGSA |
50.76 |
51.15 |
0.39 |
| 8 |
Davis, Jackson C (15)
|
NCMAC |
49.21 |
51.19 |
1.98 |
| 9 |
Gehrig, John S (15)
|
NCMAC |
52.03 |
51.41 |
-0.62 |
| 10 |
Menkhaus, Karl O (17)
|
NCMAC |
48.68 |
51.63 |
2.95 |
| 10 |
Hager, Benjamin J (15)
|
NCMAC |
53.85 |
51.63 |
-2.22 |
| 12 |
Mikuta, Reid C (16)
|
NCSGSA |
51.70 |
51.69 |
-0.01 |
| 13 |
Root, Hunter E (18)
|
NCMAC |
49.40 |
51.95 |
2.55 |
| 14 |
Hershfeld, John E (17)
|
NCWAVE |
49.53 |
51.96 |
2.43 |
| 15 |
Watson, William C (15)
|
NCSTAR |
50.64 |
51.99 |
1.35 |
| 16 |
Atherton, Alexander R (15)
|
NCMAC |
52.52 |
52.10 |
-0.42 |
| 17 |
Dorsel, Evan M (16)
|
NCMAC |
53.90 |
53.15 |
-0.75 |
| 18 |
Lucht, Samuel J (16)
|
NCMOR |
51.28 |
53.16 |
1.88 |
| 19 |
Dockham, Michael S (17)
|
NCSTAR |
49.57 |
53.23 |
3.66 |
| 20 |
Charrette, Connor B (16)
|
NCMAC |
51.11 |
53.90 |
2.79 |
| 21 |
Carter, Jared L (15)
|
NCWAVE |
51.63 |
54.05 |
2.42 |
| 22 |
Storie, Jonathan S (16)
|
NCMAC |
51.45 |
54.73 |
3.28 |
| 23 |
Egan, William A (17)
|
NCMAC |
50.60 |
55.87 |
5.27 |
| 24 |
Monteith, Canaan B (17)
|
NCMAC |
53.58 |
57.19 |
3.61 |
|
Eudy, Alexander R (16)
|
NCMAC |
48.41 |
NS |
- |
|
Wachendorfer, Jonathan T (18)
|
NCSTAR |
48.44 |
NS |
- |