| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Spears, Holly K (21)
|
ILCT |
54.87 |
55.86 |
0.99 |
| 2 |
Norris, Kiara M (16)
|
NCA |
55.43 |
57.24 |
1.81 |
| 3 |
Fanua, Lili M (17)
|
MDMETR |
59.94 |
1:01.27 |
1.33 |
| 4 |
Prentice, Casey H (18)
|
MDMETR |
58.84 |
1:01.68 |
2.84 |
| 5 |
Spears, Savanna L (16)
|
ILCT |
1:03.63 |
1:01.82 |
-1.81 |
| 6 |
Haynes, Sydney N (17)
|
NCMAC |
1:04.27 |
1:02.36 |
-1.91 |
| 7 |
Wheeler, AnQuniece C (16)
|
MIDRST |
1:02.86 |
1:05.83 |
2.97 |
| 8 |
Patil, Akshita D (16)
|
NCMAC |
1:03.06 |
1:05.86 |
2.80 |
| 9 |
Carter, Chandler C (17)
|
NJMDST |
1:00.62 |
1:06.20 |
5.58 |
| 10 |
Woods, Adanne E (15)
|
MIDRST |
1:07.40 |
1:06.74 |
-0.66 |
| 11 |
Martin, Cadence A (15)
|
NCASTF |
1:07.47 |
1:07.62 |
0.15 |
| 12 |
Johnson, Alexandra M (15)
|
VAECAT |
1:02.95 |
1:07.65 |
4.70 |
| 13 |
Ball, Shelby F (15)
|
PVTBSC |
1:07.19 |
1:10.57 |
3.38 |
| 14 |
Fielder, Mia L (18)
|
MIDRST |
1:09.09 |
1:12.70 |
3.61 |
| 15 |
Pflieger, Zoe E (17)
|
MDBCSC |
1:14.19 |
1:17.92 |
3.73 |
| 16 |
Yard, Janai N (15)
|
PVTBSC |
1:28.12 |
1:25.93 |
-2.19 |
|
Mansfield, Sarah (16)
|
PVDMV |
1:00.20 |
NS |
- |
|
Bravo, Eliya (17)
|
NJMDST |
1:19.46 |
NS |
- |
|
Toxtil, Nicole I (18)
|
NJBGCD |
1:12.76 |
NS |
- |