| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Vadapalli, Bhargav S (12)
|
NCSAIL |
40.33 |
40.13 |
-0.20 |
| 2 |
Bundy, Samuel O (12)
|
NCMSA |
43.98 |
40.43 |
-3.55 |
| 3 |
Green, Mason J (12)
|
NCQCD |
44.30 |
41.60 |
-2.70 |
| 4 |
Clark, Joseph F (12)
|
NCATOM |
|
42.42 |
- |
| 5 |
Sterka, Jackson R (12)
|
NCSAIL |
44.52 |
43.08 |
-1.44 |
| 6 |
Goins, George F (11)
|
NCTEAM |
45.93 |
43.35 |
-2.58 |
| 7 |
Henage, Charles C (11)
|
NCUN-0 |
45.56 |
43.43 |
-2.13 |
| 8 |
Wu, Philip (12)
|
NCMSA |
47.19 |
44.40 |
-2.79 |
| 9 |
Schlueter, Harmon B (12)
|
NCSAIL |
49.01 |
45.11 |
-3.90 |
| 10 |
Cureton, Christian I (12)
|
NCSAIL |
48.02 |
45.25 |
-2.77 |
| 11 |
Picard, Matthew C (12)
|
SCSWIM |
46.89 |
45.68 |
-1.21 |
| 12 |
Maxey, Stone H (12)
|
NCEAC |
48.06 |
46.03 |
-2.03 |
| 13 |
Fagan, Milo K (11)
|
NCUN-0 |
47.59 |
46.66 |
-0.93 |
| 14 |
Holtham, Isaac O (11)
|
NCMSA |
|
46.95 |
- |
| 15 |
Rosenbloom, Joshua W (12)
|
NCMSA |
|
47.41 |
- |
| 16 |
Shulgin, Nick (11)
|
NCSAIL |
49.35 |
48.10 |
-1.25 |
| 17 |
McCartan, Brodie S (11)
|
NCSAIL |
48.58 |
48.32 |
-0.26 |
| 18 |
Cooper, Christopher B (12)
|
NCMSA |
50.36 |
48.84 |
-1.52 |
| 19 |
Gibbs, Mason J (12)
|
NCSAIL |
47.34 |
49.64 |
2.30 |
| 20 |
Engel, Connor J (12)
|
NCATOM |
51.53 |
49.86 |
-1.67 |
| 21 |
Guenther, Daniel B (11)
|
NCATOM |
49.45 |
49.96 |
0.51 |
| 22 |
Harper, James W (11)
|
NCATOM |
58.28 |
50.01 |
-8.27 |
| 23 |
Halbach, Jake M (12)
|
NCSAIL |
51.24 |
50.04 |
-1.20 |
| 24 |
DiMartino, Beau T (12)
|
NCMSA |
49.38 |
50.10 |
0.72 |
| 25 |
Scott, Luke M (11)
|
NCMSA |
50.73 |
50.70 |
-0.03 |
| 26 |
Zhang, Kelvin Z (11)
|
NCMSA |
52.61 |
51.71 |
-0.90 |
| 27 |
Simmons, Nelson A (11)
|
NCATOM |
54.45 |
51.78 |
-2.67 |
| 28 |
Annas, Avery M (11)
|
NCLENR |
50.06 |
51.89 |
1.83 |
| 29 |
Fletcher, Alden N (11)
|
NCTEAM |
|
52.00 |
- |
| 30 |
Spinner, Wiley J (11)
|
NCMSA |
1:10.27 |
52.96 |
-17.31 |
| 31 |
Campbell, William L (12)
|
NCATOM |
|
52.98 |
- |
| 32 |
Hohnbaum, Nathan W (11)
|
NCATOM |
52.65 |
53.65 |
1.00 |
| 33 |
Greene, Mark L (12)
|
NCMSA |
50.93 |
54.76 |
3.83 |
| 34 |
Fowler, Luke P (12)
|
NCMSA |
1:02.59 |
1:02.76 |
0.17 |
| 35 |
Henage, Teague G (12)
|
NCUN-0 |
1:03.39 |
1:07.48 |
4.09 |
| 36 |
Reasoner, Cardyn D (11)
|
NCXA |
|
1:10.21 |
- |
| 37 |
Burgess, Dawson L (11)
|
NCCVAC |
1:15.41 |
1:13.79 |
-1.62 |
|
Clark, Owen D (11)
|
NCNSS |
1:02.08 |
DQ |
- |
|
Mogray, Joseph M (11)
|
NCCVAC |
57.19 |
NS |
- |
|
Dolan, Luke M (11)
|
NCSAIL |
1:02.67 |
NS |
- |
|
Dong, Brian (11)
|
NCMSA |
|
NS |
- |