| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Teague, Helena R (11)
|
NCSTAR |
34.84 |
34.22 |
-0.62 |
| 2 |
Tipton, Tamra N (12)
|
NCMSA |
33.08 |
34.34 |
1.26 |
| 3 |
Manning, Skylar R (12)
|
NCSTAR |
|
35.14 |
- |
| 4 |
Bryant, Kara E (12)
|
NCSTAR |
54.96 |
37.66 |
-17.30 |
| 5 |
Carmichael, Madison A (11)
|
SEGPAC |
42.05 |
41.80 |
-0.25 |
| 6 |
Williams, Christabella (12)
|
NCAQTC |
|
42.30 |
- |
| 7 |
Palmer, Sarah M (12)
|
NCSTAR |
43.19 |
44.18 |
0.99 |
| 8 |
Leventhal, Sydney F (12)
|
NCYBAC |
44.69 |
44.55 |
-0.14 |
|
Wootton, Ashlin V (12)
|
NCNSS |
36.22 |
NS |
- |
|
Goodman, Mary C (12)
|
NCSTAR |
37.39 |
NS |
- |
|
Stevens, Emily L (12)
|
NCSTAR |
43.41 |
NS |
- |