| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Tipton, Tamra N (12)
|
NCMSA |
42.20 |
43.52 |
1.32 |
| 2 |
Leventhal, Sydney F (12)
|
NCYBAC |
45.62 |
44.94 |
-0.68 |
| 3 |
Wootton, Ashlin V (12)
|
NCNSS |
46.66 |
45.92 |
-0.74 |
| 5 |
Goodman, Mary C (12)
|
NCSTAR |
46.74 |
48.08 |
1.34 |
| 7 |
Cress, Annabella J (12)
|
NCYBAC |
49.35 |
49.09 |
-0.26 |
| 8 |
Teague, Helena R (11)
|
NCSTAR |
58.53 |
49.56 |
-8.97 |
| 9 |
Gambill, Lily H (12)
|
NCPAC |
|
49.73 |
- |
| 10 |
Williams, Christabella (12)
|
NCAQTC |
|
50.65 |
- |
| 11 |
Manning, Skylar R (12)
|
NCSTAR |
53.50 |
51.44 |
-2.06 |
| 12 |
Palmer, Sarah M (12)
|
NCSTAR |
52.76 |
52.29 |
-0.47 |
| 13 |
Stevens, Emily L (12)
|
NCSTAR |
54.35 |
52.74 |
-1.61 |
| 14 |
Heilig, Lucy K (11)
|
NCTWA |
54.03 |
54.52 |
0.49 |
| 15 |
Carmichael, Madison A (11)
|
SEGPAC |
1:00.69 |
56.28 |
-4.41 |