| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Klamfoth, Avery R (9)
|
NCMAC |
46.40 |
41.04 |
-5.36 |
| 2 |
McCarter, Macie H (9)
|
NCMAC |
44.39 |
41.50 |
-2.89 |
| 3 |
Hertzig, Madison R (9)
|
NCMAC |
48.51 |
41.69 |
-6.82 |
| 4 |
Wimberly, Georgia C (9)
|
NCMAC |
43.79 |
42.46 |
-1.33 |
| 5 |
Mayers, Kelsey D (9)
|
NCMAC |
50.50 |
43.40 |
-7.10 |
| 6 |
Carlson, Sawyer G (9)
|
NCMAC |
|
45.34 |
- |
| 7 |
Holden, Mallory V (9)
|
NCMAC |
47.56 |
45.93 |
-1.63 |
| 8 |
Klein, Taylor J (9)
|
NCMAC |
47.03 |
46.19 |
-0.84 |
| 9 |
Albury, Caitlin A (9)
|
NCMAC |
|
46.24 |
- |
| 10 |
Bernardo, Clara J (9)
|
NCMAC |
|
47.21 |
- |
| 11 |
D'Amico, Sophia K (9)
|
NCMAC |
|
47.31 |
- |
| 12 |
Chia, Mollie J (9)
|
NCMAC |
53.11 |
49.09 |
-4.02 |
| 13 |
Pou, Julia M (9)
|
NCMAC |
|
49.22 |
- |
| 14 |
Bednar, Reese K (9)
|
NCMAC |
52.26 |
50.03 |
-2.23 |
| 15 |
Light, Madelyn C (9)
|
NCMAC |
|
51.17 |
- |
| 16 |
Quirk, Katherine E (9)
|
NCMAC |
|
52.23 |
- |
| 17 |
Smith, Karlin G (9)
|
NCMAC |
|
52.96 |
- |
| 18 |
Magner, Caragh M (9)
|
NCMAC |
|
53.03 |
- |
|
Ferraro, Lindsay S (9)
|
NCMAC |
|
NS |
- |
|
McConnell, Ainsley E (9)
|
NCMAC |
|
NS |
- |
|
Unnikrishnan, Radhika (9)
|
NCMAC |
|
NS |
- |