| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Kenney, Leo M (10)
|
NCTAC |
34.99 |
34.42 |
-0.57 |
| 2 |
Eickhoff, Christopher J (10)
|
NCGFSC |
36.12 |
35.33 |
-0.79 |
| 3 |
Patkar, Aneesh A (9)
|
NCTAC |
41.02 |
41.07 |
0.05 |
| 4 |
Trexler, Grant M (9)
|
NCUN-0 |
40.88 |
41.45 |
0.57 |
| 5 |
Delos Santos, Joshua B (8)
|
NCGFSC |
42.53 |
41.65 |
-0.88 |
| 6 |
Rothchild, Brennan T (9)
|
NCTAC |
45.74 |
42.45 |
-3.29 |
| 7 |
Rabon, Matthew R (9)
|
NCGFSC |
42.32 |
42.86 |
0.54 |
| 8 |
Tolba, Ali H (10)
|
NCTAC |
49.22 |
44.79 |
-4.43 |
| 9 |
Sonntag, Flynn E (9)
|
NCTAC |
45.20 |
45.33 |
0.13 |
| 10 |
Ray, Jacob T (10)
|
NCTAC |
47.69 |
45.79 |
-1.90 |
| 11 |
Benzing, William D (9)
|
NCTAC |
43.95 |
45.87 |
1.92 |
| 12 |
Torsone, Bryce G (9)
|
NCGFSC |
44.56 |
46.49 |
1.93 |
| 13 |
Yun, Matthew R (9)
|
NCTAC |
46.19 |
46.52 |
0.33 |
| 14 |
Aberant, Thomas A (10)
|
NCGFSC |
47.97 |
46.70 |
-1.27 |
| 15 |
Ruhlin, Benjamin G (9)
|
NCTAC |
48.87 |
47.27 |
-1.60 |
| 16 |
Cho, Owen N (9)
|
NCTAC |
48.47 |
47.70 |
-0.77 |
| 17 |
Kim, Lucas B (7)
|
NCTAC |
49.58 |
50.00 |
0.42 |
| 18 |
Zhao, Caden W (7)
|
NCTAC |
|
51.57 |
- |
| 19 |
Cao, Ethan (10)
|
NCTAC |
|
52.41 |
- |
| 20 |
Forrest, Robert T (9)
|
NCTAC |
|
53.53 |
- |
| 21 |
Peng, Alistair (6)
|
NCTAC |
54.87 |
54.08 |
-0.79 |
| 22 |
Shah, Ishaan B (8)
|
NCTAC |
|
54.88 |
- |
| 23 |
DeMoss, Nathan T (7)
|
NCTAC |
50.87 |
56.37 |
5.50 |
| 24 |
Solomon, Jonathan (9)
|
NCTAC |
55.87 |
1:02.64 |
6.77 |
| 25 |
Cheung, Caleb Z (7)
|
NCGFSC |
1:10.11 |
1:17.68 |
7.57 |
|
Vershinskii, Dmitrii (6)
|
NCTAC |
|
DQ |
- |
|
Said, Yusef (9)
|
NCTAC |
43.60 |
NS |
- |
|
Derwin, Nathaniel T (10)
|
NCGFSC |
36.61 |
NS |
- |