| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Duprey, James M (8)
|
NCYOTA |
42.67 |
39.74 |
-2.93 |
| 2 |
Zhang, Chuanxi (8)
|
NCYOTA |
44.47 |
42.08 |
-2.39 |
| 3 |
Wehe, Isaiah J (8)
|
NCGCY |
47.91 |
48.25 |
0.34 |
| 4 |
Slaughter, Anderson H (8)
|
NCGCY |
|
53.79 |
- |
| 5 |
Simmons, Roman S (7)
|
NCGCY |
|
56.68 |
- |
| 6 |
Caress, Baxter L (7)
|
NCYOTA |
1:00.09 |
57.59 |
-2.50 |
| 7 |
Bakelaar, Lucas A (8)
|
NCYOTA |
1:03.16 |
1:00.69 |
-2.47 |
|
Banko, Landon K (8)
|
NCYOTA |
|
DQ |
- |
|
Lange, Colby R (6)
|
NCYOTA |
1:24.09 |
DQ |
- |
|
Olson, Hugh H (6)
|
NCYOTA |
|
DQ |
- |
|
Reschke, Gavin P (8)
|
NCYOTA |
1:05.87 |
DQ |
- |
|
Williamson IV, Calvin E (8)
|
NCYOTA |
1:00.94 |
DQ |
- |
|
Beasley, Jaxon C (8)
|
NCYOTA |
1:30.03 |
NS |
- |